Pdf upconversion luminescence with tunable lifetime in nayf4. The wellknown green hot band from the2h 112 f 4i152 transition in the 520 540 nm region and stokes emissions were investigated. Based on this upconversion fluorescent nanoprobe, the detection limit of acriflavine can reach up to 0. Nirresponsive dna hybridization detection by high efficient. Optimization of a green synthesis of cdsezns nanocrystals. Multicolor polystyrene nanospheres tagged with upconversion. Pdf upconversion nanocrystals mediated lateralflow.
Upconversion photoluminescence can eliminate problems associated with autofluorescence and scattered excitation light in homogeneous luminescencebased assays without need for temporal resolution. The effects of the nanocrystal size on the emission spectra and decay rates of upconverting hexagonal nayf4. Gold and hairpin dna functionalization of upconversion. Theoretically, most lanthanide ions can undergo the nirtovisible upconversion process. We have read this dissertation and recommend that it be approved. This sensing system relies on the strong quenching of the green uc.
The upconversion fluorescence of nanoparticles is effectively quenched in the presence of epi via a fluorescence resonance energy transfer mechanism. Upconversion nanoparticlesencoded hydrogel microbeadsbased. Upconversion nanoparticlebased forster resonance energy. Unlike the upconverting nanocrystals previously reported in the literature that emit visible blue green red upconversion fluorescence, these asprepared nanoparticles emit strong nearinfrared nir, 831 nm upconversion luminescence under 980 nm excitation. Green upconversion nanocrystals for dna detection article in chemical communications 2424. The amplitude of the bisignate wave in the circular dichroism spectra of sidebyside assemblies demonstrates excellent linearity with the amount of target dna.
Dec 29, 2017 fluorescently encoded microbeads are in demand for multiplexed applications in different fields. Our results show that a small nanocrystal size, or large savol ratio increases the decay rate, in particular, the green luminescence decay rate. The test mechanism of ucl biosensor for cerbb2 oncogene. Pdf upconversion phosphors ucps that are free from. Sensitivity is the key in optical detection of lowabundant analytes, such as circulating rna or dna. Anisotropic functionalization of upconversion nanoparticles. Yber upconversion nanoparticles ucnps conjugated with. Considering that, upconversion nanoparticles are ideal candidate for development of nanosensors in biological applications as they exhibit high photostability, great emission intensity, and most importantly, being exciting by the nir irradiation. Onepot synthesis and strong nearinfrared upconversion. Comparatively, the upconversion green luminescence of email protectedce6catctpp group is more pronounced overlapping with the mitochondrial red signal than that of email protectedce6catoh group, exhibiting the superiority of ctppmodified nanoparticles over the nonmodification samples for targeting and aggregating in mitochondria matrix. Sensing using rareearthdoped upconversion nanoparticles ncbi. Green upconversion nanocrystals for dna detection request pdf. And the blue, green, and red upconversion emissions. Lanthanidedoped upconversion nanobioprobes for invitro.
Paperbased dna detection using lanthanidedoped liyf4. Effects of upconversion nanoparticles on polymerase chain. Pdf despite recent achievements to reduce surface quenching in nayf4. Inorganic upconversion uc nanocrystals doped with rareearth ions have garnered significant attentions in literature due to their potential use in color displays shalav et al. Gold nanoparticlebased colorimetric sensors for detection of. Euba nanocrystals were synthesized by a hydrothermal method. Key laboratory of analytical chemistry for biology and medicine ministry of education, college of chemistry and molecular sciences, wuhan university, wuhan 430072 p. Ultrasensitive detection of ebola virus oligonucleotide. A critical evaluation of their performance and potential in bioimaging.
By combining magneticfieldassisted bioseparation and concentration technology with magnetic nanoparticles, green upconversion fluorescence nanocrystals had been applied for the sensitive detection of dna fig. A general strategy to enhance upconversion luminescence in. Here, we propose a luminescence scheme of assay consisting of bagdf5. Upconversion nanoparticles ucnps based on nayf 4 nanocrystals with strong upconversion luminescence are synthesized by the solvothermal method. Current advances in lanthanidedoped upconversion nanostructures for detection and bioapplication. Lanthanidedoped nanocrystals that can efficiently upconvert infrared radiation into visible light are being studied with a view to applying them in the display, dnadetection and bioimaging areas. Lanthanidedoped nanocrystals that can efficiently upconvert infrared radiation into visible light are being studied with a view to applying them in the display, dna detection and bioimaging areas. Dnamediated excitonic upconversion fret switching iopscience.
A novel sensitive dna bioassay using lanthanidedoped liyf4 upconversion nanocrystals as luminescent marker and oligonucleotide hybridization as the selective reaction is developed in a paper. Therefore, the detection of dna methylation with high sensitivity and specificity is important, and various detection methods have been developed. Biosensing platform based on fluorescence resonance energy transfer from upconverting nanocrystals to graphene oxide. Apr 21, 2016 a upconversion luminescene biosensor based on dualsignal amplification for the detection of short dna species of cerbb2 oncogene. Barrett eichler department of chemistry, augustana college, sioux falls, sd 57197 two synthetic methods of cadmium selenidezinc sulfide cdsezns coreshell quantum dots qds were explored. Updownconversion luminescence rareearth iondoped y 2 o 3 1d. Oligonucleotide sensor based on selective capture of.
Single nanocrystal sensitivity achieved by enhanced upconversion. As a result, the upconversion photoluminescence can be modulated by utilizing the reversible absorption character of the. Rapid, sensitive dna test to identify human papillomavirus type 16. The change in the optical transmission surrounding the upconversion nanocrystals can reversibly quench the green upconversion emission figure 6b. These two isomers exhibit different optical absorption spectra. Now xiaojie xue and coworkers from the toyota technological. Plasmonenhanced upconversion luminescence on vertically. Exonuclease iiiassisted upconversion resonance energy. In this work, we present a simple yet versatile concept on magnifying upconversion luminescence of nayf4. Fe 2 o 3 magnetite nanoparticle, were modified with capture dna and.
Rare earth re doped upconversion nanoparticles ucnps are promising for. Osa enhanced upconversion fluorescence and altered particle. Upconversion nayf4 nanoparticles for size dependent cell. Qd based biomarker have achieved significant progress in realtime detection and bioimaging, they. Here, we developed a new multiplexed detection system.
The limit of detection for dna using sidebyside assemblies is as low as 3. Dna detection down to 10 nm was made possible by this. It is easy to derive the concentration of acriflavine from the integral intensity ratio of green emission from acriflavine to blue emission from ucnps fluorescent signals. For example, the efficient luminescence resonant energy transfer lret can be achieved by controlling the distance between nayf 4. By combining magneticfieldassisted bioseparation and concentration technology with magnetite nanoparticles, novel green upconversion uc fluorescence nanocrystals nayf 4. Research article upconversion nayf nanoparticles for size. This results in a strong polarization rotation that matches theoretical expectations. Lanthanidedoped upconversion nanobioprobes for invitro detection of tumor markers. A upconversion luminescene biosensor based on dualsignal amplification for the detection of short dna species of cerbb2 oncogene. Detection of early primary colorectal cancer with upconversion luminescent npbased molecular probes chunyan liu,a yifei qi,b ruirui qiao,a yi hou,a kaying chan,b ziqian li,b jiayi huang,a lihong jing,a jun dub and mingyuan gaoa early detection and diagnosis of cancers is extremely bene. In recent decades, various upconversion nanoparticles ucnps have been.
Nanoparticles nps are attractive materials owing to their physical and electrochemical properties, which make them extremely useful in diagnostic applications. A upconversion luminescene biosensor based on dualsignal. Ucnps are a type of inorganic nanocrystals comprised of a host matrix. Nucleic acids detection based on upconversion nanoparticles ucnps, which display a high signaltonoise ratio and no. By combining magneticfieldassisted bioseparation and concentration technology with magnetite nanoparticles, novel green upconversion uc fluorescence. The key for lethal virus detection is high sensitivity, since earlystage detection of virus may increase the probability of survival. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Coreshell structured upconversion nanocrystaldendrimer. Herein, a method to robustly disperse upconversion nanoprobes in physiological buffers based on rational design and synthesis of nanoconjugates comprising hairpin. Ebola outbreaks are currently of great concern, and therefore, development of effective diagnosis methods is urgently needed. Comparison of upconversion spectra of the assynthesised nayf 4. Green upconversion nanocrystals for dna detection chemical. Despite the general usefulness and obvious improvement of the thermal coprecipitation method for ucnps synthesis, it suffers from the longtime and continuous operation of the experimental process.
Express 5, 092601 2012 lanthanidedoped nanocrystals that can efficiently upconvert infrared radiation into visible light are being studied with a view to applying them in the display, dna detection and bioimaging areas. Expanding the toolbox for lanthanidedoped upconversion. These nayf 4 upconversion nanocrystals can be employed as fluorescence donors to pump fluorescent organic molecules. Photon upconversion is the phenomenon where highenergy photons are emitted upon excitation of lowenergy photons. Wang l y and li y d 2006 green upconversion nanocrystals for dna detection chem. Wang l and li y 2006 green upconversion nanocrystals for dna detection chem. We developed a sensor for the detection of specific microrna mirna sequences that was based on graphene quantum dots gqds and email protected the proposed sensor exploits the interaction between the sp2 carbon atoms of the gqd, mainly stacking, and the dna nucleobases anchored on the upconversion nanoparticles ucnps. The inset shows the integral intensity of green and red emission as a function of x. The emission color of these nayf 4 upconversion nanoparticles can be easily modulated by the doping. This approach imparts the upconversion nanoprobes with excellent biocompatibility and circumvents the problem of particle agglomeration.
We have demonstrated a luminescence resonance energytransferbased assay utilizing inorganic upconverting upc lanthanide phosphor as a donor and fluorescent protein as an acceptor. This dissertation, written by pingping liang, and entitled gold nanoparticlebased colorimetric sensors for detection of dna and small molecules, having been approved in respect to style and intellectual content, is referred to you for judgment. Multicolor luminescent rareearth iondoped y2o3 nanocrystals ncs were prepared. Upconversion luminescence and magnetic turning of naluf4. To date, realtime adjusting the emitting color with high brightness remains a formidable challenge. The enzyme exonuclease iii exo iii is a useful tool in this regard. Lower limits of detection may be further achieved if the detection background of autofluorescence can be removed. The influence of nanocrystal size is represented in terms of the surface areavolume ratio savol.
Recently, upconversion nanoparticles ucnps have been found to display a high signaltonoise ratio and no photobleaching, making them useful for diagnostic applications. Intense and colortunable upconversion through 980 and. Sensing using rareearthdoped upconversion nanoparticles. By combining magneticfieldassisted bioseparation and concentration technology with magnetite nanoparticles, novel green upconversion uc fluorescence nanocrystals nayf4. Upconversion nanocrystals mediated lateralflow nanoplatform for in vitro detection.
Upconversion nayf 4 nanoparticles for size dependent cell imaging and concentration dependent detection of rhodamine b shiganghu, 1 xiaofengwu, 1 zhijuntang, 1 zaifangxi, 1 zenghuichen, 2 panhu, 1 yiyu, 1 huanyuanyan, 3 andyunxinliu 2 school of information and electrical engineering, hunan university of science and technology, xiangtan, china. Optimization of a green synthesis of cdsezns nanocrystals and their bioconjugation to dna abby malmanger, lexie mohror, anna tims, and dr. Development and applications of upconversion nanoparticle in. A nearinfrared nir responsive dna probe has been developed for dna hybridization detection with characteristics of high efficient forster resonance energy transfer fret from small. Compared to organic dyebased commercially available luminexs xmap technology, upconversion nanoparticles ucnps are better alternatives due to their large antistokes shift, photostability, nil background, and single wavelength excitation. His idea was to develop an infrared photon detector using. Plasmonic modulation is a promising way to solve this bottleneck. Homogeneous assay technology based on upconverting. Key laboratory of design and assembly of functional nanostructures of chinese academy of sciences, fujian key laboratory of nanomaterials, fujian institute of research on the structure of matter, chinese academy of sciences, fuzhou 350002, china.
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